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DESCRIPTION
This product contains a single population of 6-µm particles. Every particle contains a fluorophore that is excited at 670 nm and emits at 750 nm.
The particles are supplied in 1.5 mL of water containing 0.05% Tween® 20 and 2 mM sodium azide.
RESOURCES & TOOLS
Spectrum Viewer
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PREPARATION AND STORAGE
Store vials at 2–8°C. Protect from direct light. Each reagent is stable for the period shown on the bottle label when stored as directed.
Chase ES, Hoffman RA.Resolution of dimly fluorescent particles: a practical measure of fluorescence sensitivity.Cytometry. 1998;33:267-279.
Establishing optimum baseline PMT gains to maximize resolution on BD Biosciences digital flow cytometers.BD application note. 23-8359-00.
Hoffman RA.Standardization and quantitation in flow cytometry. In: Darzynkiewicz Z, Crissman HA, Robinson JP, eds.Methods in Cell Biology. Elsevier Inc; 2001;63:300-340.
Hoffman RA.Standardization, calibration, and control in flow cytometry. Revised. In: Robinson JP, et al, eds.Current Protocols in Cytometry. New York, NY: John Wiley and Sons, Inc; Unit 1.3.
Steen HB. Noise, sensitivity, and resolution of flow cytometers.Cytometry. 1992;13:822-830.
Wood JC, Hoffman RA. Evaluating fluorescence sensitivity on flow cytometers: an overview.Cytometry. 1998;33:256-259.